Improvement of the pharmacopoeial identification of burr marigold herb by macro- and microscopic characteristics

Aim. To improve the national monograph of burr marigold (Bidens tripartita L.) herb on macro- and microscopic characteristics raw material. achieve this goal, a comparative macroscopic analysis various species an extended all parts plant that are part material were carried out. Materials methods. The study objects 7 samples medicinal Bidens L. identified registered at premises State Enterprise “Pharmacopoeia Center”. They provided by domestic manufacturers; sample whole B. frondosa collected in Akhtyrsky district ofthe Sumy region; cernua pharmaceutical enterprise. was harvested period budding beginning flowering. dry wassubject to analysis. performed powder sifted through sieve with hole diameter 355 since according requirements European Pharmacopoeia Ukraine standardized procedure for determining diagnostic signs microscopy. Results discussion. As result analysis, it has been found is mixture or fragmented stems leaves, anthodia, achenes. Stems green greenish-purple, round-oval, longitudinally grooved. Leaves brownish-green, dentateserrated edge, opposite, 3-5-parted lamina lanceolate lobes narrowed into short, slightly winged petioles. Inflorescences erect their width almost equal height. Fruits flattened achenes, apex 2 3 awns, twice as short achene. awns lateral ribs achenes covered bristles, which tips directed downwards. powder, diagnosticsigns have found, namely: fragments veins, along secretory channels reddish-brown contents pass; from upper lower epidermis basic cells sinuous walls, stomata anomocytic type, covering caterpillar form trichomes 9–18 thin-walled cells, rarely thick-walled 2-13 cells; stem rectangular elongated thickened walls; inner leaves involucres articulated branched channels; tubular flowers polygonal rounded papillary outgrowths; pollen grains spinous exine; testa pigmented darkbrown hypodermis adjacent layer yellowish sclereids. Conclusions. sections “Identification A B” (macro- material) “Burr herbN” SPhU 2.2 improved, allows more accurately identify additions developed included 2.5 text herbN”.